Cellular regulators of infectious agents and methods of use

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/12 (2006.01) A61K 31/7088 (2006.01) A61P 31/14 (2006.01) C07K 14/47 (2006.01) C12N 9/00 (2006.01) C12N 15/11 (2006.01) C12Q 1/68 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2409219

The invention provides ribozymes with target recognition sequences that allow the ribozyme to target and cleave cellular regulators that are involved in HCV and other viral replication. Also provided are nucleic acids encoding various cellular regulators and sequences in such nucleic acid for which ribozymes can be directed. Fragments of these nucleic acid and protein sequences also are provided. Further provided is a method for identifying a ribozyme reactive with a cellular regulator of viral replication or expression, and a method for identifying the cellular regulator targeted by such ribozymes. Also provided is a method of identifying a compound that modulates the activity of a cellular regulator. Further provided is a method of treating an HCV infection.

L'invention concerne des ribozymes contenant des séquences de reconnaissance de cible permettant au ribozyme de cibler puis de couper des régulateurs cellulaires impliqués dans une réplication du virus de l'hépatite C (HCV) ou d'autre virus. Elle concerne aussi des acides nucléiques codant pour différents régulateurs cellulaires, des séquences dans de tels acides nucléiques pour lesquelles des ribozymes peuvent être dirigés, ainsi que des fragments des ces séquences d'acides nucléiques et de protéine. Elle concerne aussi une méthode d'identification d'un ribozyme réactif avec un régulateur d'expression ou de réplication virale, une méthode d'identification du régulateur cellulaire ciblé par de tels ribozymes, ainsi qu'une méthode d'identification d'un composé qui module l'activité d'un régulateur cellulaire. Elle concerne enfin une méthode de traitement d'une infection par HCV.

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