Cellulase enzyme based method for the production of alcohol...

C - Chemistry – Metallurgy – 12 – P

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C12P 19/02 (2006.01) B01D 3/00 (2006.01) C07H 3/02 (2006.01) C12P 7/10 (2006.01) C12P 19/00 (2006.01) C13K 1/02 (2006.01)

Patent

CA 2694875

A process for the production of glucose and ethanol from a pretreated lignocellulosic feedstock is provided. The method comprises enzymatically hydrolyzing the pretreated lignocellulosic feedstock with cellulase enzymes to produce a hydrolyzate slurry comprising glucose and unhydrolyzed cellulose and fermenting the hydrolyzate slurry in a fermentation reaction to produce a fermentation broth comprising alcohol. A process stream is obtained comprising unhydrolyzed cellulose, which is then subjected to a denaturing step, preferably comprising exposing the unhydrolyzed cellulose to elevated temperatures, thereby producing a heat-treated stream comprising the unhydrolyzed cellulose. The heat-treated stream comprising unhydrolyzed cellulose is then further hydrolyzed with cellulase enzymes to hydrolyze the cellulose to glucose.

L'invention porte sur un procédé qui permet de produire du glucose et de l'éthanol à partir d'une charge lignocellulosique prétraitée. Ledit procédé consiste : à soumettre la charge lignocellulosique prétraitée à une hydrolyse enzymatique par des enzymes cellulases afin d'obtenir une suspension d'hydrolysat comprenant du glucose et de la cellulose non hydrolysée; et à faire fermenter la suspension d'hydrolysat lors d'une réaction de fermentation afin d'obtenir un jus de fermentation comprenant de l'alcool. On obtient alors un flux de traitement comprenant de la cellulose non hydrolysée, que l'on soumet à une étape de dénaturation consistant de préférence à exposer la cellulose non hydrolysée à des températures élevées afin de produire un flux traité thermiquement comprenant de la cellulose non hydrolysée. On soumet ensuite le flux traité thermiquement comprenant de la cellulose non hydrolysée à une hydrolyse supplémentaire par des enzymes cellulases afin d'hydrolyser la cellulose en glucose..

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