Cellulase fusion protein and heterologous cellulase fusion...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/62 (2006.01) C12N 9/42 (2006.01) C12P 21/02 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2560588

The present invention relates to a heterologous cellulase fusion construct, which encodes a fusion protein having cellulolytic activity comprising a first catalytic domain derived from a fungal exo-cellobiohydrolase and a second catalytic domain derived from a cellulase enzyme. The invention also relates to vectors and fungal host cells comprising the heterologous cellulase fusion construct as well as methods for producing said cellulase fusion protein and enzymatic cellulase compositions.

L'invention concerne une construction de fusion cellulase hétérologue codant une protéine de fusion présentant une activité cellulolytique, comprenant un premier domaine catalytique issu d'une exo-cellobiohydrolase fongique et un deuxième domaine catalytique issu d'une enzyme cellulase. L'invention concerne également des vecteurs et des cellules hôtes fongiques comprenant ladite construction de fusion cellulase hétérologue, ainsi que des procédés de production de cette protéine de fusion cellulase et des compositions cellulase enzymatiques.

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