Cellulase, method for producing the same and use thereof

C - Chemistry – Metallurgy – 12 – N

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C12N 9/42 (2006.01) C11D 3/386 (2006.01) D21C 5/00 (2006.01) D21C 9/10 (2006.01)

Patent

CA 2047613

28 ABSTRACT OF THE DISCLOSURE A cellulase having the following properties: (1) an optimum pH of from 9.5 to 10.5 as measured using carboxymethylcellulose as a substrate; (2) a stable pH of from 6 to 11 as measured using carboxymethylcellulose as a substrate after treating at 30°C and for 30 minutes; (3) an optimum temperature of about 55°C as measured using carboxymethylcellulose as a substrate; (4) influence of a surfactant being such that residual activity is 90 % or more after treatment at 30°C and pH of 9.0 for 2 hours in the presence of a sodium n-alkylbenzenesulfonate; (5) a molecular weight of 52,000 ? 2,000 as measured by electrophoresis on SDS-polyacrylamide gel; and (6) an isoelectric point of 4.2 + 0.2 as measured by isoelectric electrophoresis. The cellulase is produced by Bacillus sp. SD402, its mutant strains or its genetic engineered strains. An aid for detergents and a paper treating agent, respectively, contain the cellulase as an effective ingredient.

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