Cellulolytic enzyme gene and use thereof

C - Chemistry – Metallurgy – 12 – N

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C12N 15/09 (2006.01) C12N 9/04 (2006.01) C12N 9/42 (2006.01) C12P 1/02 (2006.01) D21C 3/00 (2006.01) D21C 5/00 (2006.01)

Patent

CA 2477175

A method for treating woodchips, comprising the steps of: preparing a DNA encoding an antisense RNA substantially complementary to the whole or a part of a transcription product of a cellulolytic enzyme gene derived from Basidiomycete; preparing a vector comprising (a) the above DNA, or (b) a recombinant DNA comprising the above DNA and a DNA fragment having a promoter activity, wherein the above DNA binds to the above DNA fragment such that an antisense RNA of the cellulolytic enzyme gene is generated as a result of transcription; transforming host cells with the above vector, so as to prepare the host cells having a suppressed cellulolytic enzyme activity; and inoculating the above host cells having a suppressed cellulolytic enzyme activity into woodchips to treat them.

Un procédé de traitement de copeaux de bois comprend une étape de préparation d'un ADN codant un ARN antisens qui est sensiblement complémentaire au produit de transcription entier d'un gène de digestion de la cellulose provenant d'un basidiomycète ou d'une partie de ce dernier; une étape de construction d'un vecteur qui contient (a) l'ADN précité ou (b) un ADN de recombinaison contenant ledit ADN et un fragment d'ADN ayant une activité de promoteur, ledit ADN étant lié au fragment d'ADN de telle sorte que l'ARN antisens de l'enzyme de digestion de la cellulose soit formé par la transcription de l'ADN; une étape de transcription effectuée à l'aide du vecteur, suivie de la préparation de cellules hôtes ayant une activité d'enzyme de digestion de la cellulose supprimée; et l'inoculation des copeaux avec les cellules hôtes ayant une activité d'enzyme de digestion de la cellulose supprimée pour traiter ainsi les copeaux de bois.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Cellulolytic enzyme gene and use thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cellulolytic enzyme gene and use thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cellulolytic enzyme gene and use thereof will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-2066419

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.