C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/56 (2006.01) G06F 19/22 (2011.01) A21D 8/04 (2006.01) A23C 9/12 (2006.01) A23K 1/165 (2006.01) A23L 1/305 (2006.01) A23L 2/66 (2006.01) A61K 38/47 (2006.01) A61L 2/16 (2006.01) C02F 3/00 (2006.01) C07H 21/00 (2006.01) C07K 16/40 (2006.01) C07K 19/00 (2006.01) C11D 3/386 (2006.01) C11D 7/42 (2006.01) C12N 5/16 (2006.01) C12N 9/24 (2006.01) C12N 9/42 (2006.01) C12N 9/98 (2006.01) C12N 11/00 (2006.01) C12N 15/00 (2006.01) C12N 15/11 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01) C12P 7/10 (2006.01
Patent
CA 2637013
The invention provides polypeptides having any cellulolytic activity, e.g., a cellulase activity, a endoglucanase, a cellobiohydrolase, a beta-glucosidase, a xylanase, a mannanse, a .beta.-xylosidase, an arabinofiiranosidase, and/or an oligomerase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having any cellulolytic activity, e.g., a cellulase activity, e.g., endoglucanase, cellobiohydrolase, beta-glucosidase, xylanase, mannanse, .beta.-xylosidase, arabinofuranosidase, and/or oligomerase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides having an oligomerase activity, e.g., enzymes that convert recalcitrant soluble oligomers to fermentable sugars in the saccharification of biomass. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts. The invention also provides compositions or products of manufacture comprising mixtures of enzymes comprising at least one enzyme of this invention.
L'invention concerne des polypeptides ayant une activité cellulolytique quelconque, par exemple une activité de cellulase, d'endoglucanase, de cellobiohydrolase, de .beta.-glucosidase, de xylanase, de mannase, de .beta.-xylosidase, d'arabinofuranosidase et/ou d'oligomérase, des polynucléotides codant pour ces polypeptides, et des procédés de préparation et d'utilisation de ces polynucléotides et polypeptides. Dans un aspect, l'invention concerne des polypeptides ayant une activité cellulolytique quelconque, par exemple une activité de cellulase, d'endoglucanase, de cellobiohydrolase, de .beta.-glucosidase, de xylanase, de mannase, de .beta.-xylosidase, d'arabinofuranosidase et/ou d'oligomérase, y compris une activité thermostable et thermotolérante, des polynucléotides codant pour ces enzymes, ainsi que la préparation et l'utilisation de ces polynucléotides et polypeptides. Dans un aspect, l'invention concerne des polypeptides ayant une activité d'oligomérase, par exemple des enzymes qui convertissent des oligomères solubles récalcitrants en sucres fermentescibles dans la saccharification de biomasse. Les polypeptides de l'invention peuvent être utilisés dans les contextes pharmaceutiques, agricoles, agro-alimentaires et industriels les plus divers. L'invention concerne également des compositions ou des produits manufacturés comprenant des mélanges d'enzymes comprenant au moins une enzyme de la présente invention.
Cayouette Michelle
Gray Kevin A.
Zhao Lishan
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
Verenium Corporation
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