Centrifugal microfluidic system for nucleic acid sample...

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Details

G01N 1/28 (2006.01) B81B 7/00 (2006.01) G01N 1/42 (2006.01) G01N 1/44 (2006.01) C12M 1/38 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) C40B 60/00 (2006.01)

Patent

CA 2717939

A microfluidic system for processing a sample includes a microfluidic CD in the form a rotatable disc, the disc containing a plurality of separate lysis chambers therein. A magnetic lysis blade and lysis beads are disposed in each of the lysis chambers and a plurality of stationary magnets are disposed adjacent to and separate from the microfluidic CD. The stationary magnets are configured to magnetically interact with each of the magnetic lysis blades upon rotation of the microfluidic CD. Each lysis chamber may have its own separate sample inlet port or, alternatively, the lysis chambers may be connected to one another with a single inlet port coupled to one of the lysis chambers. Downstream processing may include nucleic acid amplification using thermoelectric heating as well as detection using a nucleic acid microarray.

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