A - Human Necessities – 61 – B
Patent
A - Human Necessities
61
B
A61B 10/00 (2006.01) B01L 3/14 (2006.01) C12M 1/00 (2006.01) C12M 3/00 (2006.01) C12Q 1/24 (2006.01) C12N 5/08 (2006.01) C12N 5/06 (2006.01)
Patent
CA 2198606
Disclosed is a centrifugable cell separation apparatus that is useful in density gradient separation of cells and other biological materials. In a specific embodiment, the apparatus takes the form of a syringe (10) that allows for the withdrawal of a sample and which contains a movable plunger (18) containing a constricted region (28) that restricts mixing between pellet and supernatant fractions following centrifugation. The syringe (10) may also contain a density gradient separation solution (20). Also disclosed are a kit and a method for extracting a fluid specimen that utilise the apparatus.
L'invention concerne un dispositif de séparation de cellules par centrifugation permettant d'effectuer la séparation de cellules et d'autres matériaux biologiques en fonction de leur gradient de densité. Dans un mode de réalisation spécifique, ce dispositif se présente sous la forme d'une seringue (10) permettant de prélever un spécimen et contenant un plongeur mobile (18) comportant une zone rétrécie (28) limitant le mélange entre les fractions granulaires et les fractions surnageantes après la centrifugation. La seringue (10) peut également contenir une solution de séparation par gradient de densité (20). L'invention concerne également un kit et un procédé d'extraction d'un spécimen de fluide utilisant ledit dispositif.
Activated Cell Therapy Inc.
Dendreon Corporation
Gowling Lafleur Henderson Llp
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