Centrifuging apparatus and method for separation of liquid...

B - Operations – Transporting – 01 – J

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B01J 19/00 (2006.01) B04B 5/04 (2006.01) C07K 1/04 (2006.01) G01N 35/00 (2006.01) G01N 35/02 (2006.01)

Patent

CA 2361223

A simple, efficient apparatus and method for separating layers of immiscible or partially miscible liquids useful in methods of high-throughput combinatorial organic synthesis or parallel extraction of large libraries or megaarays of organic compounds is disclosed. The method comprises: (a) positioning a plurality of reaction vessels containing a liquid or mixture of liquids in a holder on the perimeter of a centrifuge rotor in a tilted position with a tilt away from the axis of rotation; and (b) spinning the rotor of the centrifuge at a speed so that the liquid is expelled from said vessels. In a preferred embodiment, an apparatus and method for separating layers of immiscible or partially miscible liquids compatible with microtiter plate type array(s) of reaction vessels is disclosed. Another application of centrifugation based liquid removal was found for washing the plates in biological assays or synthesis on modified substrates.

La présente invention concerne un appareil simple, efficace, et un procédé destinés à la séparation de couches de liquides immiscibles ou partiellement miscibles, utiles dans des procédés de synthèse organique combinatoire à haute productivité ou dans l'extraction parallèle de grandes banques ou de méga-matrices de composés organiques. Cet appareil et cette méthode sont utiles, comme partie d'un système automatique, robotique ou manuel de synthèse organique combinatoire ou de purification (extraction). Dans une réalisation préférée, l'invention concerne un appareil et un procédé destinés à séparer des couches de liquides immiscibles ou partiellement miscibles, compatibles avec des réseaux du type plaque à microtitration d'enceintes réactionnelles. Une autre application de l'élimination de liquide par centrifugation réside dans le nettoyage des plaques utilisées dans des tests biologiques ou dans des synthèses sur des substrats modifiés.

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