Cephalosporin acetylhydrolase gene and protein encoded by...

Details Cephalosporin acetylhydrolase gene and protein encoded by... Cephalosporin acetylhydrolase gene and protein encoded by...

C - Chemistry, Metallurgy

12

N

530/13, 195/33.1

C12N 15/70 (2006.01) C12N 1/21 (2006.01) C12N 9/18 (2006.01) C12N 15/55 (2006.01) C12P 35/00 (2006.01)

Patent

CA 2040707

The invention relates to a cephalosporin acetylhydrolase gene, a recombinant DNA molecule prepared by introducing the gene into a vector used in an E. coli host-vector system, E. coli cells transformed with the recombinant DNA molecule, a protein having the amino acid sequence encoded by the gene, an enzyme which is a multimer of the protein, and a process for preparing the protein or enzyme. The cephalosporin acetylhydrolase is an enzyme useful for converting cephalosporins, e.g. cephalosporin C and 7-ACA, into deacetylated ones, e.g. deacetylcephalosporin C and deacetyl 7-ACA, which are useful as intermediates in the preparation of a variety of derivatized cephalosporin antibiotics.

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