Cephalosporin c acylase

Details Cephalosporin c acylase Cephalosporin c acylase

C - Chemistry, Metallurgy

12

N

C12N 15/55 (2006.01) C12N 1/21 (2006.01) C12N 9/18 (2006.01) C12N 9/80 (2006.01) C12P 35/04 (2006.01) C12P 35/06 (2006.01)

Patent

CA 2051090

There is described a cephalosporin C acylase having the following characteristics: (a) it has the ability to catalyze the enzymatic conversion of cephalosporin C, glutaryl 7-ACA, adipyl 7-ACA, succinyl 7-ACA, N-acetylcephalosporin C, N-benzoyl-cephalosporin C and cephalothin into 7-amino cephalo-sporanic acid, (b) it is composed of .alpha.-subunit [molecular weight: 26,000 dalton (SDS-PAGE)] and .beta.-subunit [molecular weight: 58,000 dalton (SDS-PAGE)], (c) it has the following N-terminal amino acid sequence of the .alpha.-subunit: Thr-Met-Ala-Ala-Asn- Thr-Asp-Arg-Ala-Val-Leu-Gln-Ala-Ala-Leu-Pro-Pro-Leu-. A DNA which encodes cephalosporin C acylase is also described, as well as an expression vector which comprises this DNA and a host cell transformed by the expression vector. Also described is a process of preparing the above cephalosporin C acylase. This product has higher stability and higher enzymatic potency.

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