C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/54 (2006.01) A21D 8/04 (2006.01) C12N 9/10 (2006.01)
Patent
CA 2529209
The inventors have developed a method of modifying the amino acid sequence of a CGTase to obtain variants. The variants may form linear oligosaccharides as an initial product by starch hydrolysis and a reduced amount of cyclodextrin and may be useful for anti-staling in baked products. The method is based on a comparison of three-dimensional (3D) structures of the CGTase with the structure of a maltogenic alpha-amylase where one or both models includes a substrate. The invention also provides novel CGTase variants.
La présente invention concerne un procédé permettant de modifier la séquence d'acides aminés d'une CGTase pour obtenir des variantes. Ces variantes peuvent former des oligosaccharides linéaires comme produit initial sous l'effet de l'hydrolyse de l'amidon et une quantité réduite de cyclodextrine et peuvent être utilisées comme agent anti-rassissement dans les produits boulangers. Ce procédé repose sur une comparaison de structures tridimensionnelles (3D) de la CGTase avec la structure d'une alpha-amylase maltogénique, un ou les deux modèles comprenant un substrat. Cette invention concerne également de nouvelles variantes de CGTase.
Beier Lars
Jensen Morten Tovborg
Jorgensen Christel Thea
Spendler Tina
Svendsen Allan
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
Novozymes A/s
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