Chain length specific udp-glc:fatty acid glucosyltransferases

Details Chain length specific udp-glc:fatty acid glucosyltransferases Chain length specific udp-glc:fatty acid glucosyltransferases

C - Chemistry, Metallurgy

12

N

C12N 9/10 (2006.01) C12P 7/62 (2006.01) C12P 19/02 (2006.01)

Patent

CA 2300366

Enzyme activities which transfer glucose from uridine 5'-diphosphate glucose to fatty acids to form 1-O-acyl-.beta.-glucoses which act as acyl donors in the esterification of glucose and further esterification of partially acylated glucose and in the esterification of sucrose and further esterification of partially acylated sucrose, are separated according to specificity for transferring glucose to short, medium or long chain length fatty acids. DNA molecules coding for the enzyme activities are isolated. Methods for preparing 1-O-acyl-.beta.-D-glucoses comprise reacting uridine 5'-diphosphate glucose and fatty acid in the presence of the appropriate enzyme activity.

Cette invention concerne des activités enzymatiques qui transfèrent le glucose de l'uridine de 5-diphosphate glucose sur des acides gras pour former des 1-O-acyle-â-glucoses qui agissent en tant que donneurs d'acyle dans l'estérification du glucose puis dans l'estérification du glucose partiellement acylé et dans l'estérification du saccharose et ensuite dans l'estérification du saccharose partiellement acylé. On sépare ces activités enzymatiques en fonction de la spécificité pour transférer le glucose sur des acides gras à chaîne courte, moyenne ou longue. Des molécules d'ADN codant les activités enzymatiques sont isolées. Des procédés permettant de préparer des 1-O-acyle-â-D-glucoses consistent à faire réagir de l'uridine 5-diphosphate glucose et un acide gras en présence de l'activité enzymatique appropriée.

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