Chain-termination type nucleic acid sequencing method...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2238297

A chain-termination type DNA sequencing method is disclosed wherein deoxynucleotide 2'-deoxythymidine-5'-triphosphate replaced by 2'-deoxyuridine-5'-triphosphate, or analogs thereof. Kits for performing the method are also provided.

L'invention concerne un procédé de séquençage d'ADN par la méthode de SANGER, dans lequel le désoxynucléotide 2'-désoxythymidine-5'-triphosphate est remplacé par du 2'-déoxyuridine-5'-triphosphate ou par des analogues de ce dernier. L'invention concerne également des kits pour réaliser ledit procédé.

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