Characterising nucleic acids

Details Characterising nucleic acids Characterising nucleic acids

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2295955

A method for characterising DNA, which comprises: (i) providing a population of DNA fragments, each fragment having cleavably attached thereto a mass label for identifying a feature of that fragment; (ii) separating the fragments on the basis of their length; (iii) cleaving each fragment in a mass spectrometer to release its mass label; and (iv) determining each mass label by mass spectroscopy to relate the feature of each fragment to the length of the fragment.

L'invention porte sur un procédé de caractérisation d'ADN, ce procédé consistant à: (i) produire une population de fragments d'ADN, chaque fragment étant lié de manière clivable à une étiquette de masse de façon à identifier une caractéristique de ce fragment; (ii) séparer les fragments sur leur longueur; (iii) cliver chaque fragment dans un spectromètre de masse pour libérer son étiquette; et (iv) déterminer chaque étiquette par spectrométrie de masse pour associer la caractéristique de chaque fragment à sa longueur.

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