C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/0735 (2010.01) C12N 5/071 (2010.01) C12Q 1/04 (2006.01) G01N 33/569 (2006.01) A61K 35/12 (2006.01)
Patent
CA 2466342
A sub-population of HES cells is identified which is positive for specific HES markers. These cell types are identified by expression of particular GCTM-2 antigens. A method based on the identification of GCTM-2 can differentiate between substantially undifferentiated or differentiated cell populations. Spontaneous differentiation often occurs in HES cultures resulting in non- homogeneous cell cultures. The invention also provides further sub populations of cells within differentiated cultures of HES cells that are SCL or epithelial in nature.
Selon l'invention, une sous-population de cellules HES qui sont positives en ce qui concerne des marqueurs spécifiques HES est identifiée. Ces types de cellules sont identifiées au moyen de l'expression d'antigènes GCTM-2 particuliers. Une méthode basée sur l'identification de GCTM-2 permet de différencier des populations de cellules sensiblement non différenciées ou différenciées. Une différentiation spontanée se produit souvent dans les cultures de HES, donnant lieu à des cultures cellulaires non homogènes. L'invention concerne également d'autres sous-populations de cellules dans des cultures différenciées de cellules HES qui sont de nature SCL ou épithéliales.
Gion Tomonobu
Hawes Susan
Laslett Andrew
Pera Martin Frederick
Es Cell International Pte Ltd
Sim & Mcburney
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