Characterization of n-glycan mixtures by nuclear magnetic...

G - Physics – 01 – N

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G01N 24/08 (2006.01) G01N 33/15 (2006.01) G01N 33/483 (2006.01) G01R 33/46 (2006.01)

Patent

CA 2682746

The present disclosure provides nuclear magnetic resonance (NMR) methods for characterizing mixtures of N-linked glycans. Without limitation, methods of the present disclosure may be useful in characterizing monosaccharide composition, branching, fucosylation, sulfation, phosphorylation, sialylation linkages, presence of impurities and/or efficiency of a labeling procedure (e.g., labeling with a fluorophore such as 2-AB). In certain embodiments, the methods can be used quantitatively. In certain embodiments, the methods can be combined with enzymatic digestion to further characterize glycan mixtures.

La présente invention concerne des méthodes de résonance magnétique nucléaire (RMN) destinées à la caractérisation de mélanges de N-glycanes. Sans restrictions, les méthodes de la présente invention peuvent être utiles pour la caractérisation de la composition des monosaccharides, des liaisons de ramification, de fucosylation, de sulfatation, de phosphorylation, de sialylation, la présence d'impuretés et/ou le rendement d'un processus de marquage (un marquage à l'aide d'un fluorophore tel que 2-AB, par exemple). Dans certains modes de réalisation, les méthodes peuvent être utilisées de manière quantitative. Dans d'autres modes de réalisation, les méthodes peuvent être combinées avec une digestion enzymatique pour une caractérisation plus précise des mélanges de glycanes.

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