Characterization of n-glycans using exoglycosidases

Details Characterization of n-glycans using exoglycosidases Characterization of n-glycans using exoglycosidases

G - Physics

01

N

G01N 33/68 (2006.01)

Patent

CA 2682695

The present disclosure provides methods for analyzing structure and/or composition of N-glycans. Such methods often involve digestion of N-glycans with multiple exoglycosidases. In some embodiments, N-glycans are digested with multiple exoglycosidases simultaneously. In some embodiments, N-glycans are digested with multiple exoglycosidases sequentially. In some embodiments, methods in accordance with the present disclosure involve comparison of cleavage products of N-glycans that have been digested with multiple exoglycosidases simultaneously to N-glycans that have been digested with multiple exoglycosidases sequentially.

La présente invention concerne des méthodes permettant d'analyser la structure et/ou la composition de N-glycanes. Ces méthodes comprennent souvent la digestion de N-glycanes par plusieurs exoglycosidases. Dans certains modes de réalisation, les N-glycanes sont digérés par plusieurs exoglycosidases, simultanément. Dans d'autres modes de réalisation, les N-glycanes sont digérés par plusieurs exoglycosidases, en séquence. Dans certains autres modes de réalisation, les méthodes selon la présente invention comprennent une comparaison entre des produits de clivage de N-glycanes digérés par plusieurs exoglycosidases, simultanément, et de N-glycanes digérés par plusieurs exoglycosidases, en séquence.

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