C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01)
Patent
CA 2483489
A novel method for characterizing nucleic acids. A nucleic acid is combined with a double stranded nucleic acid-specific dye to form a detectable complex between the dye and one or more double stranded structures within the nucleic acid. The combination is then exposed to varying temperatures and the fluorescence emission of the dye is measured to determine the melting temperature(s) for the double stranded structures. In some embodiments that melting temperature profile is then compared to melting temperature profiles generated for other nucleic acid(s) to discern differences between the compared nucleic acids.
La présente invention concerne un nouveau procédé de caractérisation d'acides nucléiques. Selon l'invention, on combine un acide nucléique avec un colorant spécifique d'acide nucléique double brin afin de former un complexe détectable entre le colorant et une ou plusieurs structures à double brin au sein de l'acide nucléique. On expose ensuite la combinaison à des températures variables et l'on mesure l'émission de fluorescence du colorant afin de déterminer la ou les températures de fusion des structures à double brin. Dans certains modes de réalisation, on compare alors le profil des températures de fusion obtenu avec des profils de températures de fusion obtenus pour un ou plusieurs autres acides nucléiques afin de déceler des différences entre les acides nucléiques comparés.
Dummer Charles W.
Wittwer Carl T.
Smart & Biggar
University Of Utah Research Foundation
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