Charge tags and the separation of nucleic acid molecules

Details Charge tags and the separation of nucleic acid molecules Charge tags and the separation of nucleic acid molecules

C - Chemistry, Metallurgy

07

H

C07H 21/00 (2006.01) C07F 9/24 (2006.01) C07F 9/59 (2006.01)

Patent

CA 2436746

The present invention relates to novel phosphoramidites, including positive and neutrally charged compounds. The present invention also provides charge tags for attachment to materials including solid supports and nucleic acids, wherein the charge tags increase or decrease the net charge of the material. The present invention further provides methods for separating and characterizing molecules based on the charge differentials between modified and unmodified materials.

L'invention porte sur de nouvelles phosphoramidites, comprenant des composants à charge positive ou neutre, des marqueurs de charge à fixer à des matériaux dont des supports solides, et des acides nucléiques, lesdits marqueurs augmentant ou réduisant la charge nette des matériaux. L'invention porte également sur des procédés de séparation et de caractérisation de molécules se basant sur les différentiels de charge entre les matériaux modifiés et non modifiés.

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