Chemical blocking agent against non-specific binding or...

Details Chemical blocking agent against non-specific binding or... Chemical blocking agent against non-specific binding or...

G - Physics

01

N

167/43, 167/45

G01N 33/53 (2006.01) G01N 33/554 (2006.01) G01N 33/573 (2006.01)

Patent

CA 1305920

ABSTRACT A chemical agent is provided for significantly preventing or blocking non-specific staining or binding of an antibody specific for Terminal Deoxynucleotydal Transferase (TdT) during immunofluorescent or immunoperoxidase assay procedures. These procedures include both immunofluorescent and immunohistochemi- cal staining of samples followed by flow cytometric and/or mi- croscopic analysis, respectively. The invention is practiced by selective use of casein introduced into the assay procedures at an appropriate interval prior to analysis using a labeled or tagged monoclonal antibody specific to a TdT epitope. The casein utilized successfully was obtained from a large variety of sources and includes the use of a non-fat milk product. -1-

546443

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