Chemical induction of quiescence in bacteria

C - Chemistry – Metallurgy – 12 – N

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C12N 1/20 (2006.01) C12N 15/67 (2006.01)

Patent

CA 2633456

Quiescence is induced in cells using indole compounds. Expression continues from extra-chromosomal vectors within the cells during quiescence, while chromosomal expression is suppressed. The cells may be used as factories for the production of large amounts of polypeptides of interest, particularly polypeptides which normally have an adverse effect on cell viability or growth. Expression from an extra-chromosomal vector of interest may be monitored, in view of the reduced background expression from the chromosome. Vector copy number may be amplified. Cell cycles may be synchronised.

La quiescence est induite dans des cellules en utilisant des composés d'indole. L'expression se poursuit à partir de vecteurs extrachromosomiques à l'intérieur des cellules quiescentes tandis que l'expression chromosomique est supprimée. Les cellules peuvent être utilisées comme des usines de production de grandes quantités de polypeptides d'intérêt, en particulier des polypeptides qui ont normalement un effet néfaste sur la viabilité ou la croissance des cellules. L'expression à partir d'un vecteur extrachromosomique d'intérêt peut être contrôlée en vue de réduire l'expression de fond à partir du chromosome. Le nombre de copies du vecteur peut être amplifié. Les cycles cellulaires peuvent être synchronisés.

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