Chemical modification of dna using peptide nucleic acid...

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A01N 43/04 (2006.01) A01N 63/00 (2006.01) A61K 48/00 (2006.01) C07K 14/00 (2006.01) C07K 14/035 (2006.01) C12N 5/00 (2006.01) C12N 13/00 (2006.01) C12N 15/65 (2006.01) C12N 15/67 (2006.01)

Patent

CA 2303908

Complexes comprising a nucleic acid molecule and a conjugated peptide nucleic acid (PNA). The PNA may be labeled or conjugated to a protein, peptide, carbohydrate moiety or receptor ligand. These complexes are used to transfect cells to monitoring plasmid biodistribution, promote nuclear localization, induce transcriptional activation, lyse the endosomal compartment and facilitate transfection. Theses complexes increase the efficiency of expression of a particular gene.

La présente invention concerne des complexes comprenant une molécule d'acide nucléique et un acide nucléique peptidique conjugué (ANP). L'APN peut être marqué ou conjugué à une protéine, un peptide, une fraction de glucide ou un ligand de récepteur. Ces complexes sont utilisés pour transfecter des cellules, pour surveiller une biodistribution de plasmide, pour promouvoir un emplacement nucléaire, pour indure une activation transcriptionnelle, pour lyser le compartiment endosomal et pour faciliter une transfection. Ces complexes augmentent l'efficacité d'une expression d'un gène particulier.

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