Chemical proteomics

G - Physics – 01 – N

Patent

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Details

G01N 33/94 (2006.01) G01N 30/72 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/553 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2474469

The invention relates to methods and reagents for identifying/isolating protein targets of chemical compounds (for example, drug candidates) using mass spectrometry. The invention provides a method for capturing and identifying proteins using tethered small-molecule probes. This technology also allows the market expansion of known drugs by finding new therapeutic targets; identification of the mechanism of toxicity of drug candidates or drugs which failed in the clinic; identification of new chemical tools for chemically-driven target validation; identification of new drug leads; and identification of the mechanism of action of drugs and drug candidates. A key advantage of the technology is that a single experiment can identify the numerous proteins which interact with a probe (or "bait").

L'invention concerne des procédés et des réactifs visant à identifier/isoler des protéines cibles de composés chimiques (par exemple, candidats thérapeutiques) par spectrométrie de masse. L'invention concerne un procédé de capture et d'identification de protéines à l'aide de sondes fixées à de petites molécules. Cette technique permet d'étendre le marché de médicaments connus grâce à la découverte de nouvelles cibles thérapeutiques ; d'identifier le mécanisme de toxicité de candidats thérapeutiques ou de médicaments ayant échoué en phase clinique ; d'identifier de nouveaux outils chimiques pour la validation de cibles par des moyens chimiques ; d'identifier de nouvelles pistes thérapeutiques ; et d'identifier le mécanisme d'action de médicaments et de candidats thérapeutiques. Un avantage clé de cette technique consiste en ce qu'elle permet d'identifier en une seule expérimentation de nombreuses protéines interagissant avec une sonde (ou "appât").

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