Chemical sample treatment cassette and methods

G - Physics – 01 – N

Patent

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Details

G01N 1/00 (2006.01) B01L 3/00 (2006.01) B01L 9/06 (2006.01) G01N 1/28 (2006.01) G01N 30/04 (2006.01) G01N 30/88 (2006.01) G01N 33/68 (2006.01) G01N 35/02 (2006.01) G01N 30/02 (2006.01) G01N 30/24 (2006.01) G01N 30/36 (2006.01) G01N 30/46 (2006.01) G01N 30/60 (2006.01) G01N 35/00 (2006.01)

Patent

CA 2259648

This invention is directed to a cassette chemical immobilization and treatment system (10) and method for enabling the performance of various complex chemistries with minimal human intervention, near-zero dead volume, and flow- through protocols pursuant to a predetermined instruction set encoded on a multiple-address chemical treatment cassette assembly. The cassette assembly (12) comprises a plurality of analyte sample columns (13) ("mini-columns"), reagent wells (31) containing pre-packaged reagents including powdered, microencapsulated, liquid or lyophillized reagents, analyte loading funnels (19), an alignment assembly (15) for identifying a chemical treatment protocol. The mini-columns (13) are improved columns having high pressure interface capability to permit direct insertion of the mini-column into a high- pressure solvent line for use as a support column for HPLC analysis.

L'invention a trait à un système de cassette (10) d'immobilisation et de traitement chimique, et à un procédé permettant l'accomplissement de fonctions chimiques complexes variées, nécessitant une intervention humaine minimale, un volume mort presque nul, et des protocoles en continu conformes à un ensemble d'instructions préétablies, codées sur un ensemble cassette de traitement chimique à fonctions multiples. L'ensemble cassette (12) comprend plusieurs colonnes (19) d'échantillons d'analytes ("mini-colonnes"), des puits à réactifs contenant des réactifs pré-conditionnés, notamment des réactifs en poudre, en microcapsules, sous forme liquide ou lyophilisés, des entonnoirs à analytes, un moyen d'alignement des colonnes d'échantillons d'analyte, et un ensemble de codes (25) lisible par machine, destiné à identifier un protocole de traitement chimique. Les mini-colonnes (19) sont des colonnes améliorées, dotées d'une fonction d'interface haute pression permettant de les insérer directement dans un conduit de solvant à haute pression, en vue de leur utilisation comme colonne support dans des chromatographies liquides à haute performance.

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