Chemical treatment of biological samples for nucleic acid...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07G 99/00 (2009.01) C07H 21/04 (2006.01) C12N 1/08 (2006.01)

Patent

CA 2515039

A composition and method for the purification of nucleic acid are disclosed. The composition includes at least one alkaline agent and at least one detergent. The composition preferably also includes a suspension of paramagnetic particles and an acidic solution. The method involves the use of the composition with paramagnetic particles to extract nucleic acid from a biological sample.

L'invention concerne une composition et une méthode de purification d'acide nucléique. Cette composition comprend au moins un agent alcalin et au moins un détergent. De préférence, ladite composition comprend également une suspension de particules paramagnétiques et une solution acide. Ledit procédé consiste à utiliser cette composition avec les particules paramagnétiques pour extraire un acide nucléique à partir d'un échantillon biologique.

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