Chemically excitable fluorescer-labeled specific binding assay

G - Physics – 01 – N

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167/43

G01N 33/52 (2006.01) G01N 33/533 (2006.01)

Patent

CA 1133392

ABSTRACT OF THE DISCLOSURE A specific binding assay method and reagent means for determining a ligand, such as an antigen or antibody, in, or the ligand binding capacity of, a liquid medium which employs a fluorescent substance, i.e. a fluorescer, as a label. The improvement comprises measuring the fluorescer-label by chemically exciting the label and measuring the resulting light emitted thereby. Chemical excitation of the label is preferably accomplished by exposure to a substance, such as a high energy intermediate produced by the reaction between hydrogen peroxide and highly reactive materials such as oxalyl chloride, oxamides and bis-oxalate esters. The assay may follow conventional homogeneous and heterogeneous formats. The improved assay is more convenient than conven- tional fluorescent binding assays by obviating the need for photogenic excitation of the fluorescent label.

341540

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