Chemically modified oligonucleotide primers for nucleic acid...

C - Chemistry – Metallurgy – 12 – P

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C12P 19/34 (2006.01) C07H 21/00 (2006.01) C07H 21/04 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2653841

The present invention provides methods and compositions for nucleic acid amplification. These methods involve the use of oligonucleotide primers in temperature dependent nucleic acid amplification reactions. In certain aspects, the methods are accomplished by use of certain modified oligonucleotide primers which provide utility in nucleic acid amplification. In preferred embodiments, the oligonucleotide primers are modified with particular chemical groups such as esters.

La présente invention présente des procédés et des compositions pour l'amplification de l'acide nucléique. Ces procédés impliquent l'utilisation d'amorces d'oligonucléotides dans des réactions thermo-dépendantes d'amplification d'acide nucléique. Dans certains cas, les procédés sont mis en oeuvre à l'aide de certaines amorces d'oligonucléotides modifiées qui présentent une utilité dans l'amplification de l'acide nucléique. Dans des modes de réalisation préférés, les amorces d'oligonucléotides sont modifiées à l'aide de groupes chimiques particuliers, tels que les esters.

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