G - Physics – 01 – N
Patent
G - Physics
01
N
150/9
G01N 33/52 (2006.01) G01N 33/50 (2006.01) G01N 33/566 (2006.01) G01N 33/569 (2006.01) G01N 33/58 (2006.01)
Patent
CA 2028605
-36- CHEMILUMINESCENCE ASSAY OF INFLAMMATION Abstract of the Invention The presence or amount of inflammation of a patient is determined by comparing the extent of opsonin receptor expression on phagocytes of a patient with the maximum opsonin receptor expression inducible on phagocytes of the patient in vitro after stimulation with a receptor expression priming agent. Preferably, the state of inflammation of a patient is determined by contacting afirst portion of a phagocyte containing biological sample from the patient with an opsonified oxidative metabolism stimulating agent capable of eliciting metabolicactivation and with a chemiluminigenic substrate, contacting a second portion ofthe biological sample from the patient with an opsonin receptor expression priming agent, an opsonified oxidative metabolism stimulating agent capable of eliciting metabolic activation and a chemiluminigenic substrate, and then comparing the chemiluminescence response of the first and second portions of thesample as a measure of the immune response potential or state of inflammation ofthe patient. Phagocyte function is additionally quantitatively evaluated by measuring the phagocyte oxygenation capacity of a maximally opsonin receptor primed and stimulated biological sample of a patient, determining the specific oxygenation capacity to a set of controls representing the normal distribution of specific oxygenation capacity established from testing a large population. The phagocyte-specific oxygenation capacity is determined by contacting the sample with an opsonin receptor expression priming agent, an opsonified oxidative metabolism stimulating agent and a chemiluminigenic substrate, measuring the chemiluminescence response of the sample, determining the chemiluminescence response per phagocyte of the sample and comparing the response per phagocyte with that of the normal range of values. Kits and reagents are provided for use in the practice of the disclosed methods. 1/EXOX-5256
Exoxemis Inc.
Goudreau Gage Dubuc
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