G - Physics – 01 – N
Patent
G - Physics
01
N
167/43, 150/15.2
G01N 33/52 (2006.01) C07C 43/196 (2006.01) C07C 69/00 (2006.01) C07D 321/00 (2006.01) C07F 9/12 (2006.01) C07F 9/655 (2006.01) C07F 9/6574 (2006.01) C07H 15/203 (2006.01) C07H 15/26 (2006.01) C12Q 1/34 (2006.01) C12Q 1/42 (2006.01) C12Q 1/68 (2006.01) G01N 21/76 (2006.01) G01N 33/531 (2006.01) G01N 33/533 (2006.01) G01N 33/535 (2006.01) G01N 33/58 (2006.01) G01N 33/76 (2006.01)
Patent
CA 1340590
Water soluble naturally-occurring and synthetic enhancer substances, generally macromolecular in nature, for example globular proteins that include hydrophobic regions such as bovine serum albumin, and polymeric quaternary ammonium salts such as poly(vinylbenzyltrimethylammonium chloride), which have the ability to inhibit light-emitting fluorophores resulting from the decomposition of chemiluminescent compounds from releasing energy through non-light emitting pathways, are disclosed as permitting the stabilization, and hence increasing the light intensity, of such light-emitting fluorophores in aqueous media as compared to the intensity of the light emitted by the same quantities of such fluorophores in aqueous media in the absence of such enhancer substances. Any chemiluminescent chemical compound, including thermally, chemically, electrochemically or enzymatically cleavable 1,2-dioxetanes, for example 3-(2'-spiroadamantane)-4-methoxy-(3"-phosphoryloxy)phenyl-1,2-dioxetane disodium salt, and acridfnium esters, can be used. Auxiliary fluorophores, for example fluorescein and derivatized fluoresceins, that accept energy from fluorophores produced by decomposition of a chemiluminescent compound and in turn emit detectable energy, can also be present. Such enhancer substance/chemiluminescent compound compositions are useful in detecting the presence or determining the concentration of chemical or biological substances in immunoassays, chemical assays and nucleic acid probe assays, and in chemical/physical probe procedures for studying the microstructures of macromolecules.
604507
Bronstein Irena Y.
Edwards Brooks
Voyta John C.
Applied Biosystems Llc
Mccarthy Tetrault Llp
Tropix Inc.
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