Chemiluminescent double-triggered 1,2-dioxetanes

Details Chemiluminescent double-triggered 1,2-dioxetanes Chemiluminescent double-triggered 1,2-dioxetanes

C - Chemistry, Metallurgy

07

H

C07H 15/26 (2006.01) C07D 321/00 (2006.01) C07F 9/655 (2006.01) C07F 9/6558 (2006.01) C07F 9/6574 (2006.01) C07H 19/10 (2006.01) C09K 11/07 (2006.01) C12Q 1/42 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2055590

A stable 1,2-dioxetane of the formula (I), where R1, R2 and R3 are passive organic groups that allow the dioxetane to decompose and produce light when Z and Y are removed, with the proviso that R1 and R2 may be joined to form a cyclic moiety, Z is a first protecting group that can be removed by a first deprotecting process, Y is a second protecting group that can be removed by a second deprotecting process, and X is an organic group which upon removal of Z and Y renders the dioxetane susceptible to said decomposition, with the proviso that X may be joined to R3 to form a cyclic moiety. These di- oxetanes are useful for generating chemiluminescent output in immunoassays and nucleic acid hybridization assays.

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