Chemiluminescent electron-rich aryl-substituted 1,2-dioxetanes

Details Chemiluminescent electron-rich aryl-substituted 1,2-dioxetanes Chemiluminescent electron-rich aryl-substituted 1,2-dioxetanes

C - Chemistry, Metallurgy

07

F

C07F 7/18 (2006.01) C07F 9/655 (2006.01) C12Q 1/34 (2006.01) G01N 33/53 (2006.01) G01N 33/58 (2006.01) C12Q 1/42 (2006.01)

Patent

CA 2146669

Chemiluminescent electron-rich aryl-substituted 1,2-dioxetane compounds are disclosed in which the aryl group is polysubstituted with suitable electron-donating groups such that the light-emitting pattern of the molecule results in a very high luminescent count, thus providing for a sensitive and precise assay for haptens, analytes, polynucleotides and the like. These substituted aryl-containing 1,2-dioxetane compounds can be used as direct labels in an immunoassay or when derivatized with an appropriate leaving group, can be used as a substrate for an enzyme immunoassay. The unusual chemiluminescence of the compounds allows the timing of the luminescent reaction to be exactly controlled.

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