Chemiluminescent energy transfer assays

G - Physics – 01 – N

Patent

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Details

G01N 33/535 (2006.01) C12Q 1/00 (2006.01) C12Q 1/68 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2212738

A chemiluminescent assays for the determination of the presence or amount of a biopolymer in bound assays using 1,2-dioxetanes in connection with AttoPhosTM as chemiluminescent substrates for enzymelabeled targets or probes is provided. Further disclosed is a kit for conducting a bioassay for the presence or concentration of a biopolymer comprising a) an enzyme complex; b) a 1,2-dioxetane; and c) AttoPhosTM.

L'invention présente des dosages par chimioluminescence permettant de déterminer la présence ou la quantité d'un biopolymère, dans des dosages par liaison superficielle utilisant des 1, 2-dioxétanes en association avec AttoPhos?TM¿ comme substrats chimiluminescents pour des cibles ou des sondes marquées par une enzyme. Elle présente, en outre, un nécessaire permettant de procéder à un dosage biologique afin de déterminer la présence ou la concentration d'un biopolymère; ce nécessaire comprend a), un complexe enzymatique, b), un 1, 2-dioxétane et, c), AttoPhos?TM¿.

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