Chemiluminescent energy transfer assays

Details Chemiluminescent energy transfer assays Chemiluminescent energy transfer assays

G - Physics

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G01N 33/53 (2006.01) C07D 321/00 (2006.01) C12Q 1/25 (2006.01) C12Q 1/34 (2006.01) G01N 21/76 (2006.01) G01N 33/52 (2006.01) G01N 33/535 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2179726

Chemiluminescent assays for the determination of the presence or amount of a biopolymer in bound assays using 1,2-dioxetanes in connection with AttoPhosTM as chemiluminescent substrates for enzymelabeled targets or probes is provided. Further disclosed is a kit for conducting a bioassay for the presence or concentration of a biopolymer comprising a) an enzyme complex; b) a 1,2-dioxetane; and c) AttoPhosTM.

L'invention concerne des dosages chimioluminescents destinés à la détermination de la présence ou de la quantité d'un biopolymère dans des dosages limités, utilisant des 1,2-dioxétanes conjointement avec de l'AttoPhos?TM¿ utilisés comme substrats chimioluminescents pour des cibles ou des sondes à marquage enzymatique. L'invention concerne également un matériel permettant de procéder à un dosage biologique déterminant la présence ou la concentration d'un biopolymère, comprenant a) un complexe enzymatique, b) un 1,2-dioxétane, et c) de l'AttoPhos?TM¿.

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