Chemiluminescent method and compositions

Details Chemiluminescent method and compositions Chemiluminescent method and compositions

C - Chemistry, Metallurgy

12

Q

C12Q 1/34 (2006.01) C09K 11/06 (2006.01) C12Q 1/68 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2061189

ABSTRACT OF THE DISCLOSURE A chemiluminescent method using a protected enhancer compound which is triggered by a hydrolytic enzyme and then enhances a chemiluminescent reaction is described. The reaction involves an amino substituted acylhydrazide which is activated to produce light by a peroxide compound and a peroxidase in the presence of the activated enhancer compound. The result is enhanced generation of light from the reaction. The hydrolytic enzyme is present alone or as a label in an immunoassay, DNA probe assay or other assay wherein the hydrolytic enzyme is bound to a reporter molecule.

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