Chemiluminescent method of monitoring products after heat...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/42 (2006.01) C12Q 1/28 (2006.01) G01N 33/04 (2006.01) G01N 33/12 (2006.01)

Patent

CA 2228290

A method of monitoring heat processes in pharmaceutical, clinical, food or non- biological products, which method includes the steps of combining a test sample or sample extract from the product with chemiluminescent compounds or compounds specifically susceptible to enzymes, while triggering the decomposition of the chemiluminescent compound to emit light. The enzyme can be indegenous or added before the heat process as an indicator. The sample is incubated and integration of chemiluminescent light emission over time is measured as indicative of the past temperature and time profile of the sample.

L'invention porte sur un procédé de contrôle de processus thermiques utilisés pour le traitement de produits pharmaceutiques, cliniques, alimentaires ou non biologiques, consistant à mélanger un échantillon ou un extrait d'échantillon d'essai du produit à tester avec des composés chimioluminescents ou des composés présentant une sensibilité spécifique à une enzyme, tout en provoquant la décomposition génératrice de lumière du composé luminescent. L'enzyme peut être indigène ou ajoutée à titre d'indicateur avant le processus thermique. L'échantillon ayant été incubé, on mesure l'intégration dans le temps de l'émission de lumière chimioluminescente qui constitue un indicateur de la température passée et du profil temporel de l'échantillon.

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