Chemiluminescent phthalhydrazide-labeled conjugates

G - Physics – 01 – N

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260/1, 260/19, 5

G01N 33/533 (2006.01) C07D 209/48 (2006.01) C07D 237/32 (2006.01)

Patent

CA 1137975

ABSTRACT OF THE DISCLOSURE Chemiluminescent-labeled conjugates of the formula: Image wherein one of R1 and R2 is hydrogen and the other is -NX3R4; R3 is hydrogen or straight chain alkyl containing 1-4 carbon atoms and R4 is Image wherein n = 2-8 and L(CO? is a specifically bindable ligand, such as an antigenic protein or polypeptide, a hapten or an antibody, or a binding ar.alog thereof, bound through an amide bond; and intermediates produced in the synthesis of such conjugates. The labeled conjugates are useful as reagents in specific binding assays for determining ligands or their specific binding partners in liquid media.

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