Chemiluminescent reagent and assay using a substituted...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/28 (2006.01) C12Q 1/68 (2006.01) G01N 33/52 (2006.01) G01N 33/53 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2157062

A simplified composition for generating a chemiluminescent signal in the presence of a peroxidase includes a substituted acetanilide as the sole light--producing substrate for the peroxidase. Assays for various analytes can be carried out with this composition at near neutral pH, exhibit low background and are highly sensitive.

Une composition simplifiée pour générer un signal chimioluminescent en présence d'une peroxydase inclut un acétanilide substitué comme le seul substrat produisant de la lumière pour la peroxydase. Des essais pour divers analytes peuvent être réalisés avec cette composition à près d'un pH neutre et présentent un faible fond et sont très sensibles.

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