C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/16 (2006.01) C07K 16/00 (2006.01) C12N 15/06 (2006.01) C12P 21/02 (2006.01)
Patent
CA 2066433
ABSTRACT OF THE DISCLOSURE A thymidine kinase-lacking hybrid cell having an Ig-producing ability in which chicken B lymphoblast with immunized chicken spleen cell, a method of obtaining the above hybrid cell which comprises mutating chicken B lymphoblast cell, isolating a thymidine kinase-lacking cell using a culture medium containing a thymidine analog, fusing it with immunized chicken spleen cell, mutating the hybrid cell, and isolating a thymidine kinase-lacking hybrid cell using a culture medium containing a thymidine analog, a hybrid cell having an IgG-producing ability in which a chicken spleen cell immunized with an antigen is further fused with the above hybrid cell, a method of producing a chicken IgG which comprises culturing the above hybrid cell having an IgG-producing ability in a culture medium. The antibody-producing cell of the invention can produce chicken IgG stably for a long period, and thereby it opens the way for the massproduction of chicken IgG.
Matsuda Haruo
Nishinaka Shigeyuki
Suzuki Takashi
Matsuda Haruo
Nishinaka Shigeyuki
Nkk Corporation
Suzuki Takashi
Swabey Ogilvy Renault
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