Chimeric animal and method for producing the same

Details Chimeric animal and method for producing the same Chimeric animal and method for producing the same

C - Chemistry, Metallurgy

12

N

C12N 5/12 (2006.01) A01K 67/027 (2006.01) C07K 16/00 (2006.01) C07K 16/18 (2006.01) C12N 15/06 (2006.01) C12N 15/08 (2006.01) C12N 15/87 (2006.01) C12N 15/90 (2006.01) C12P 21/00 (2006.01) C12P 21/08 (2006.01)

Patent

CA 2230759

A method for constructing a chimeric nonhuman animal which comprises preparing microcells containing one or more foreign chromosomes or fragments of the same, and transferring the foreign chromosome(s) or fragments of the same into cells having differentiative pluripotency via the fusion with the microcells; a chimeric nonhuman animal which can be constructed by the above method and offsprings of the same; tissues and cells originating in the same; a method for utilizing the tissues and cells; cells which contain one or more foreign chromosomes or fragments of the same and sustain differentiative pluripotency; a method for constructing the cells; and a method for utilizing the cells.

Ce procédé de constitution d'un animal chimérique consiste à préparer des micro-cellules contenant un ou plusieurs chromosomes ou fragments de chromosomes étrangers, puis à transférer ce ou ces chromosomes ou fragments dans des cellules présentant une pluripotence de différenciation, par fusion avec les microcellules. On décrit un animal chimérique, pouvant être consitué par ce procédé, ainsi que sa descendance; des tissus et cellules provenant dudit animal; un procédé d'utilisation de ces tissus et cellules; des cellules contenant un ou plusieurs chromosomes, ou fragments de chromosomes étrangers et présentant une pluripotence de différenciation; un procédé de constitution des cellules, et un procédé pour leur utilisation.

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