Chimeric bird from embryonic stem cells

C - Chemistry – Metallurgy – 12 – N

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C12N 15/00 (2006.01) C12N 15/873 (2010.01) A01K 67/027 (2006.01) C12N 15/06 (2006.01) C12N 15/85 (2006.01) C12N 5/0735 (2010.01) C12N 5/10 (2006.01)

Patent

CA 2480012

Sustained cultures of avian embryonic stem cells are provided. Injection of avian embryonic stem cells into recipient embryos yields chimeras with a significant contribution from the embryonic stem cell phenotype. Transgene encoding exogenous proteins are stably integrated in the embryonic stem cells and are present in the somatic tissue of the resulting chimeras. The transgenes may encode exogenous proteins expressed in endodermal, ectodermal, mesodermal, or extra embryonic tissue. Breeding the resulting chimera yields transgenic birds whose genome is comprised of exogenous DNA.

L'invention concerne des cultures continues de cellules souches embryonnaires aviaires. Selon l'invention, l'injection de cellules souches embryonnaires dans des embryons récepteurs permet de produire des chimères avec une contribution importante du phénotype dérivé des cellules souches embryonnaires. Des transgènes codant de protéines exogènes sont intégrés de manière stable dans lesdites cellules souches embryonnaires et sont présents dans les tissus somatiques desdites chimères résultantes. Lesdits transgènes peuvent coder des protéines exogènes exprimées dans des tissus endodermiques, ectodermiques, mésodermiques ou extra-embryonnaires. La reproduction des chimères obtenues permet de produire des oiseaux transgéniques dont le génome comprend de l'ADN exogène.

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