Chimeric delta-endotoxin expression in pseudomonas fluorescens

Details Chimeric delta-endotoxin expression in pseudomonas fluorescens Chimeric delta-endotoxin expression in pseudomonas fluorescens

C - Chemistry, Metallurgy

12

N

C12N 15/32 (2006.01) A01N 63/00 (2006.01) A01N 63/02 (2006.01) C07K 14/325 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2188795

Bacillus thuringiensis endotoxin expression in Pseudomonads can be improved by modifying the gene encoding the Bacillus thuringiensis endotoxin. Chimeric genes are created by replacing the segment of the Bacillus thuringiensis gene encoding a native protoxin with a segment encoding a different protoxin. Exemplified herein is the cryIF/cryl(b) chimera wherein the native cryIF protoxin segment has been substituted by the cryIA(b) protoxin segment, to yield improved expression of the cryIF toxin in Pseudomanads. The invention also concerns novel genes and plasmids.

L'expression de l'endotoxine du Bacillus thuringiensis dans les Pseudomonas peut être améliorée en modifiant le gène codant l'endotoxine du Bacillus thuringiensis. On crée les gènes chimères en remplaçant le segment du gène Bacillus thuringiensis codant une protoxine native par un segment codant une protoxine différente. A titre d'exemple ici, on décrit la chimère cryIF/cryI(b) dans laquelle le segment de protoxine cryIF native a été substitué par le segment de protoxine cryIA(b) pour améliorer l'expression de la toxine cryIF dans les Pseudomonas. La présente invention concerne également de nouveaux gènes et des plasmides.

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