C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/82 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C07K 14/00 (2006.01) C07K 14/415 (2006.01) C12N 9/12 (2006.01) C12N 9/88 (2006.01) C12N 15/11 (2006.01) C12N 15/60 (2006.01)
Patent
CA 2177351
This invention relates to three chimeric genes, the first encoding dihydrodipicolinic acid synthase (DHDPS), which is insensitive to inhibition by lysine and operably linked to a plant chloroplast transit sequence, a second encoding a lysine-rich protein, and a third encoding a plant lysine ketoglutarate reductase, all operably linked to plant seed-specific regulatory sequences. Methods for their use to produce increased levels of lysine in the seeds of transformed plants are provided. Also provided are transformed corn, rapeseed and soybean plants wherein the seeds accumulate lysine to higher levels than untransformed plants.
L'invention concerne trois gènes chimères, un premier codant la synthase d'acide dihydrodipicolinique (DHDPS), laquelle est insensible à l'inhibition par la lysine et liée fonctionnellement à une séquence de transit de chloroplaste végétal, un second codant une protéine riche en lysine, et un troisième codant une réductase de cétoglutarate de lysine végétal, tous liés fonctionnellement à des séquences régulatrices spécifiques à des semences végétales. L'invention concerne également leur procédé d'utilisation afin de produire des niveaux accrus de lysine dans les semences de plantes transformées. De plus, l'invention concerne du blé, du colza et du soja transformés, dans lesquels les semences accumulent la lysine à des niveaux supérieurs.
Falco Saverio Carl
Keeler Sharon Jo
Rice Janet Ann
E. I. Du Pont de Nemours And Company
Torys Llp
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