Chimeric genes and methods for increasing the lysine content...

Details Chimeric genes and methods for increasing the lysine content... Chimeric genes and methods for increasing the lysine content...

C - Chemistry, Metallurgy

12

N

C12N 9/06 (2006.01) C12N 9/12 (2006.01) C12N 9/88 (2006.01) C12N 15/82 (2006.01) C12P 13/08 (2006.01)

Patent

CA 2280196

Chimeric genes are disclosed. One chimeric gene encodes a plant lysine ketoglutarate reductase and a second chimeric gene encodes lysine-insensitive dihydrodipicolinic acid synthase (DHDPS) which is operably linked to a plant chloroplast transit sequence, all operably linked to plant seed-specific regulatory sequences. Methods for their use to produce increased levels of lysine in the seeds of transformed plants are provided.

L'invention porte sur des gènes chimères, un gène chimère codant une cétoglutarate réductase de la lysine et un second gène chimère codant une synthase d'acide dihydrodipicolinique insensible à la lysine et qui est lié de manière fonctionnelle à une séquence de transit de chloroplaste de végétaux, ces gènes étant liés de manière fonctionnelle à des séquences régulatrices spécifiques des graines de végétaux. L'invention porte également sur les procédés d'utilisation de ces gènes en vue de produire des taux supérieurs de lysine dans les graines de végétaux transformés.

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