Chimeric insecticidal protein and gene coding therefore

C - Chemistry – Metallurgy – 12 – N

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C12N 15/32 (2006.01) A01H 5/00 (2006.01) A01N 63/02 (2006.01) C07K 14/325 (2006.01) C07K 14/435 (2006.01) C12N 15/62 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2324229

The present invention describes the design and construction of a chimeric insecticidal protein by joining the 5' portion of a synthetic maize optimized cry1B gene (SFLIB) to the 3' end of a full-length synthetic maize optimized cry1A(b) gene to generate a full-length hybrid cry1B gene (hyFLIB). When the chimeric insecticidal protein gene is expressed in transgenic maize from both PEPC and pith promoters, insecticidal activity is observed in transgenic maize tissue against European corn borer (Ostrinia nubilalis). An additional aspect of the invention is recombinant, biologically pure microbial strains transformed with the hyFLIB gene which can be used in entomocidal formulations for the control of Lepidopteran insects. Yet another aspect of the invention is plants transformed with the toxin gene or active fragments thereof, particularly where the transforming sequences have been optimized for expression in maize.

La présente invention concerne la conception et la construction d'une protéine insecticide chimère, que l'on peut obtenir en reliant la partie 5' d'un gène cry1B optimisé de maïs reconstitué (SFLIB) à l'extrémité 3' d'un gène cry1A(b) entier optimisé de maïs reconstitué, de manière à produire un gène cry1B entier hybride (hyFLIB). Lorsque le gène de ladite protéine insecticide chimère est exprimé dans le maïs transgénique à partir de promoteurs PEPC et de promoteurs médullaires, on peut observer dans le tissu du maïs transgénique une activité insecticide contre la pyrale du maïs (Ostrinia nubilalis). Dans un autre aspect, cette invention concerne des souches microbiennes recombinées biologiquement pures, transformées à l'aide dudit gène hyFLIB et pouvant être utilisées dans des formulations entomocides, afin de lutter contre les lépidoptères. Cette invention concerne enfin des végétaux transformés à l'aide d'un gène de toxines, ou de fragments actifs de celui-ci, les séquences transformées étant optimisées en vue de leur expression dans le maïs.

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