Chimeric plant genes possessing independent regulatory...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01)

Patent

CA 2162925

The present invention relates to chimeric gene constructions comprising snRNA or mRNA promoter elements and 3' termination signals which are independent of the promoter element in functional combination with a structural gene which codes for a polypeptide, which is expressed, in a natural manner, from an mRNA gene, for sense RNA, for anti-sense RNA or for snRNA. In addition to this, the present invention relates to recombinant DNA molecules which encompass such chimeric gene constructs, and, in addition, to microorganisms which harbour these plasmids and to processes for preparing the said chimeric gene constructs and plasmids. Finally, the invention relates to plant cells, to parts of plants which can be regenerated to form entire and preferably fertile plants, and to the plants themselves including their descendants, insofar as they express the chimeric genes according to the invention.

La présente invention se rapporte à la production par recombinaison de gènes chimères comprenant des éléments promoteurs d'ARNsn ou d'ARNm et des signaux de terminaison 3' qui sont indépendants de l'élément promoteur en combinaison fonctionnelle avec un gène structural qui code pour un polypeptide, qui est exprimé, de manière naturelle, à partir d'un gène d'ARNm, pour l'ARN sens, pour l'ARN antisens ou pour l'ARNsn. De plus, la présente invention se rapporte à des molécules d'ADN de recombinaison qui renferment ces gènes chimères de recombinaison, et, également, à des microorganismes qui abritent ces plasmides et à des procédés de préparation des gènes chimères de recombinaison et des plasmides. L'invention se rapporte enfin à des cellules de végétaux, à des parties de végétaux qui peuvent être régénérées pour former des plantes complètes et de préférence fertiles, et aux plantes elles-mêmes y compris leurs descendants, pour autant qu'ils expriment les gènes chimères selon l'invention.

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