Chimeric plasmid vector

C - Chemistry – Metallurgy – 12 – N

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195/1.15

C12N 15/00 (2006.01) C12N 15/74 (2006.01) C12N 15/75 (2006.01)

Patent

CA 1235080

TITLE OF THE INVENTION "CHIMERIC PLASMID VECTOR" ABSTRACT OF THE DISCLOSURE A chimeric plasmid vector containing (a) a tetracycline resistance gene region (Tc) derived from the plasmid pAM.alpha.1 of Streptococcus faecalis DS5 (ATCC14508), (b) an ampicilline resistance gene region (Amp) derived from the vector pACYC177 of E. coli, (c) a first DNA replication origin (OripAM 1) derived from the plasmid pAM.alpha.1, (d) a second DNA replication origin (Oril77) derived from the vector pACYC177, and (e) a polylinker region having recognition and cleavage sites for the restriction enzymes EcoRI at one terminal and HindIII at the other terminal of the polylinker DNA sequence.

482316

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