Chimeric plasmids that replicate in bacteria and yeast and...

C - Chemistry – Metallurgy – 12 – N

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195/1.22, 195/1.

C12N 15/00 (2006.01) C12N 1/16 (2006.01) C12N 1/20 (2006.01)

Patent

CA 1205027

CHIMERIC PLASMIDS THAT REPLICATE IN BACTERIA AND YEAST AND MICROORGANISMS TRANSFORMED THEREWITH Abstract of the Invention Chimeric plasmids capable of transforming bacteria and yeast are described. The plasmids carry the Cm (chloramphenicol resistance) gene and the Tc (tetracycline resistance) gene as selectable markers. The Cm gene allows the plasmids to be selected for in wild- type strains of yeast, including Saccharomyces cerevisiae. The Tc gene allows heterologous genes cloned into the plasmids to be selected for in bacteria, including Escherichia coli.

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