Chimeric proteins for measuring atp concentrations in...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/435 (2006.01)

Patent

CA 2608852

The invention relates to luminescent chimeric proteins comprising a first N- terminal protein sequence, a second protein sequence and a third C-terminal protein sequence wherein: (i) said first and said third protein sequence are a leader sequence and an anchor sequence belonging to at least a receptor localized on a plasma membrane site; (ii) said second protein sequence encodes for the full-length or partial sequence of a photoprotein and is inserted in frame between said first and said third sequence (i); said chimeric protein being addressed to said plasma membrane site of the cell wherein it is expressed.

L'invention concerne des protéines chimériques luminescentes comprenant une première séquence protéique N-terminale, une deuxième séquence protéique et une troisième séquence protéique C-terminale. Dans ladite invention, (i) ladite première et ladite troisième séquence protéique sont une séquence de tête et une séquence d'ancrage appartenant à au moins un récepteur localisé sur un site de membrane plasmique ; (ii) ladite deuxième séquence protéique code la séquence complète ou partielle d'une photoprotéine et est insérée dans un cadre défini entre ladite première et ladite troisième séquence (i) ; ladite protéine chimérique étant adressée audit site de membrane plasmique de la cellule dans laquelle elle est exprimée.

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