Chimeric proteins, their heterodimer complexes, and platelet...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/62 (2006.01) A61K 38/17 (2006.01) A61K 47/48 (2006.01) C07K 14/705 (2006.01) C07K 16/00 (2006.01) C07K 19/00 (2006.01) C12N 15/12 (2006.01) C12N 15/13 (2006.01) G01N 33/554 (2006.01) G01N 33/566 (2006.01) G01N 33/569 (2006.01) G01N 33/68 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2250291

Integrin-immunoglobulin chimeric protein heterodimer complexes in which the .alpha.-chain and .beta.-chain of integrins have been associated in a stable state. These complexes are not only usable as drugs as such but also applicable to the assaying of binding of integrins to ligands and the detection of substances binding to integrins or those inhibiting binding of integrins to ligands. These comnplexes are also usable as diagnostic agents. It has been found that integrins isolated in a stably associated structure would bind to extracellular matrixes under physiological conditions in the presence of plasma components, which indicates that integrins and, in its turn, extracellular matrix receptors might be usable as substitutes for platelets.

L'invention concerne des complexes hétérodimères d'une protéine chimère intégrine-immunoglobuline dans lesquels la chaîne .alpha. et la chaîne .beta. des intégrines ont été associées dans un état stable. Ces complexes ne sont pas seulement directement utilisables en tant que médicaments, ils sont également utilisables pour tester la liaison d'intégrines à des ligands et pour détecter des substances se liant aux intégrines ou des substances empêchant la liaison d'intégrines à des ligands. On peut également utiliser ces complexes en tant qu'agents diagnostiques. On a découvert que les intégrines isolées dans une structure associée de manière stable se lieraient à des matrices extracellulaires dans des conditions physiologiques, en présence de composants du plasma. Ceci veut dire que les intégrines et, ensuite, les récepteurs de matrice extracellulaire peuvent être utilisés en tant que substituants de plaquettes.

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